反应详情

4-carboxyphenylthiourea (Lancaster, 13047) was dissolved to 0.2M in DMF. The resultant mixture was activated by adding a half equivalent of DIC for 15 min at room temperature. Aminopropylsilylated glass surfaces purified with compressed air (2.5×7.5 cm; Sigma, Silane-Prep™, S4651) were coated with the activated solution thus obtained and incubated for three hours at room temperature. The glass surfaces thus treated were then washed five times using respectively 30 mL of DMF for 3 minutes each at room temperature. After washing three times for three minutes each using respectively 30 mL of DCM at room temperature, the glass surfaces were dried and stored at 4° C. until further use.

1. customAminopropylsilylated glass surfaces purified with compressed air (2.5×7.5 cm; Sigma, Silane-Prep™, S4651)
2. customthus obtained
3. additionThe glass surfaces thus treated
4. washwere then washed five times
5. customfor 3 minutes
6. customat room temperature
7. washAfter washing three times for three minutes each using respectively 30 mL of DCM at room temperature
8. customthe glass surfaces were dried
9. customstored at 4° C. until further use