反应详情

A round-bottomed flask was charged with 3-fluoro-4-(2-(4-methylpiperazin-1-yl)thieno[3,2-b]pyridin-7-yloxy)aniline (40.0 mg, 0.112 mmol), 2-(4-fluorophenyl)-3-oxo-2,3-dihydropyridazine-4-carboxylic acid (39.2 mg, 0.167 mmol), N1-((ethylimino)methylene)-N3,N3-dimethylpropane-1,3-diamine hydrochloride (64.2 mg, 0.335 mmol), 1H-benzo[d][1,2,3]triazol-1-ol (45.2 mg, 0.335 mmol), N-ethyl-N-isopropylpropan-2-amine (0.0996 ml, 0.558 mmol) and DMF (10 mL). The reaction mixture was stirred at room temperature until the starting material had been consumed (overnight). Then the reaction was partitioned between EtOAc (50 mL) and H2O (50 mL). The phases were separated and the aqueous phase was re-extracted with EtOAc (3×100 mL). The combined organic layers were dried (Na2SO4), filtered and concentrated to yield a crude product. The crude product was purified by silica gel chromatography (DCM/7 M NH3 in MeOH from 100/1 to 10/1, v/v) to afford product (48.3 mg, 75.3%). LRMS (APCI pos): >99% purity, 254 nm, m/e 575 (M+1). 1H NMR (400 MHz, CDCl3) δ 11.80 (s, 1H), 8.40 (d, 1H), 8.28 (d, 1H), 8.23 (d, 1H), 7.92 (dd, 1H), 7.61 (m, 2H), 7.36 (m, 1H), 7.17-7.24 (m, 3H), 6.36 (s, 1H), 6.29 (d, 1H), 3.37 (m, 4H), 2.58 (m, 4H), 2.36 (s, 3H).

1. customhad been consumed (overnight)
2. customThen the reaction was partitioned between EtOAc (50 mL) and H2O (50 mL)
3. customThe phases were separated
4. extractionthe aqueous phase was re-extracted with EtOAc (3×100 mL)
5. dry with materialThe combined organic layers were dried (Na2SO4)
6. filtrationfiltered
7. concentrationconcentrated
8. customto yield a crude product
9. customThe crude product was purified by silica gel chromatography (DCM/7 M NH3 in MeOH from 100/1 to 10/1