反应详情

温度

50 °C

A solution of Example 146B in dioxane (3 mL) and water (0.3 mL) was treated with 3N LiOH (0.6 mL); stirred at 50° C. for 4 days, acidified with 1N HCl, treated with ethyl acetate, washed with brine (3×), dried (MgSO4), filtered, and concentrated and ethyl acetate was added. The ethyl acetate layer was washed with brine (3×), dried over magnesium sulfate anhydrous. The concentrate was purified by reverse phase chromatography to provide 1.5 mg of the desired product. MS (ESI(−)) m/e 346 (M−H)−.

1. washwashed with brine (3×)
2. dry with materialdried (MgSO4)
3. filtrationfiltered
4. concentrationconcentrated
5. additionethyl acetate was added
6. washThe ethyl acetate layer was washed with brine (3×)
7. dry with materialdried over magnesium sulfate anhydrous
8. customThe concentrate was purified by reverse phase chromatography