Hydrochloric Acid → alpha-Maltotriose

HRID 1731513Notes

Reaction equation

HRID 1731513 reaction equation
C([O-])(=O)C.[Ca+2].C([O-])(=O)C.[CH2:10]([OH:32])[C@H:11]1[O:16][C@H:15]([O:17][C@H:18]2[C@H:23]([OH:24])[C@@H:22]([OH:25])[C@H:21]([OH:26])[O:20][C@@H:19]2[CH2:27][OH:28])[C@H:14]([OH:29])[C@@H:13]([OH:30])[C@@H:12]1[OH:31].Cl.[O:34]=[CH:35][C@@H:36]([C@H:38]([C@@H:40]([C@@H:42]([CH2:44][OH:45])[OH:43])O)[OH:39])[OH:37]>C([O-])(=O)C.[Na+]>[CH2:10]([OH:32])[C@H:11]1[O:16][C@H:15]([O:17][C@H:18]2[C@H:23]([OH:24])[C@@H:22]([OH:25])[C@@H:21]([O:26][C@H:40]3[C@H:38]([OH:39])[C@@H:36]([OH:37])[C@@H:35]([OH:34])[O:43][C@@H:42]3[CH2:44][OH:45])[O:20][C@@H:19]2[CH2:27][OH:28])[C@H:14]([OH:29])[C@@H:13]([OH:30])[C@@H:12]1[OH:31]

Participants

6

Reactant 4

HCID 313Hydrochloric AcidHCID 6116Calcium AcetateHCID 6255beta-Maltose2×HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Product 1

HCID 192826alpha-Maltotriose

Reagents, catalysts & solvents 1

HCID 517045Sodium AcetateSolvent

Procedure

Further, each fraction of FG3 and FG5 was taken out and dissolved in a 0.1 M sodium acetate buffer containing 10 mM calcium acetate buffer solution (pH 5.5), followed by addition of takaamylase A for hydrolyses at 37° C. for 3 hours. The hydrolyzed products were tested by HPLC and TLC. From FG5, there were produced FG3 and maltose, but FG3 was not hydrolyzed by takaamylase A. When FG3 was added to 0.3N HCl and hydrolyzed at 100° C. for 30 minutes, the products were maltose, glucose and 2 kinds of compounds having fluorescence but no maltotriose was produced.

Workup

  1. additionfollowed by addition of takaamylase A for hydrolyses at 37° C. for 3 hours
  2. customFrom FG5, there were produced FG3

Sources & evidence

ORD record
ord-be9e9242d8fa48f5bb5e2c16ef5c2861
Source dataset
uspto-grants-1986_11
Patent number
US04622295

Reaction notes

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