反应详情

The procedure described in Comparative Example A was repeated using a 10 mL aqueous solution containing lithium L-lactate (0.750M), FMN (0.01 mM), isobutyric acid (HPLC internal standard, 0.100M), ethylenediamine (0.788M), soluble spinach glycolate oxidase (1.0 IU/mL), and soluble Aspergillus niger catalase (1,400 IU/mL) at pH 7.5 (adjusted with 50% NaOH) and the reaction run at 5° C. After 40 hours, the HPLC yields of pyruvate and acetate were 33.2% and 0%, respectively, and 22.2% lactate remained. The remaining activity of glycolate oxidase and catalase were 28% and 100%, respectively, of their initial values.

1. customat 5° C