Ethylenediamine → Acetate

HRID 1735661노트

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HRID 1735661 반응식
[C:1]([O-:6])(=[O:5])[C@H:2]([CH3:4])[OH:3].[Li+].[C:8]([OH:13])(=[O:12])[CH:9](C)C.C(N)CN.C([O-])(=O)CO.[OH-].[Na+]>>[C:1]([O-:6])(=[O:5])[C:2]([CH3:4])=[O:3].[C:8]([O-:13])(=[O:12])[CH3:9]

참가 물질

7

반응물 5

HCID 3301EthylenediamineHCID 6590Isobutyric AcidHCID 14798Sodium HydroxideHCID 5460308HydroxyacetateHCID 23687877Lithium (S)-2-hydroxypropanoate

생성물 2

HCID 175AcetateHCID 107735Pyruvate

실험 절차

The procedure described in Comparative Example A was repeated using a 10 mL aqueous solution containing lithium L-lactate (0.750M), FMN (0.01 mM), isobutyric acid (HPLC internal standard, 0.100M), ethylenediamine (0.788M), soluble spinach glycolate oxidase (1.0 IU/mL), and soluble Aspergillus niger catalase (1,400 IU/mL) at pH 7.5 (adjusted with 50% NaOH) and the reaction run at 5° C. After 40 hours, the HPLC yields of pyruvate and acetate were 33.2% and 0%, respectively, and 22.2% lactate remained. The remaining activity of glycolate oxidase and catalase were 28% and 100%, respectively, of their initial values.

후처리

  1. customat 5° C

출처 및 근거

ORD 기록
ord-04cc5b07333445e793952d32d61e1e10
출처 데이터셋
uspto-grants-1996_07
특허 번호
US05538875

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