Hydrochloric Acid → Tricalcium Phosphate

Temperature

22 °C

Combine in a suitable vessel, 2.1M KCl (35 mL), 0.0175M CaCl2 (5 mL), 0.01M KH2PO4 (50mL), and de-ionized water (350 mL). A standard pH electrode equipped with a Standard Calomel Reference electrode is inserted and the temperature adjusted to 37° C. while purging of the solution of oxygen. Once the temperature and pH are stabilized, a solution of the crystal growth inhibitor to be test is then added. A typical inhibitor test concentration is 1×10−6 M. The solution is titrated to pH 7.4 with 0.05M KOH. The mixture is then treated with 5 mL's of a hydroxyapatite slurry. The hydroxyapatite slurry can be prepared by digesting Bio-Gel® HTP hydroxyapatite powder (100 g) in 1 L of distilled water the pH of which is adjusted to 2.5 by the addition of sufficient 6N HCl and subsequently heating the solution until all of the hydroxyapatite is dissolved (heating for several days may be necessary). The temperature of the solution is then maintained at about 22° C. while the pH is adjusted to 12 by the addition of a solution of 50% aqueous KOH. Once again the solution is heated and the resulting slurry is allowed to settle for two days before the supernatant is removed. 1.5 L of distilled water is added, the solution stirred, then after settling again for 2 days the supernatant is removed. This rinsing procedure is repeated six more time after which the pH of the solution is adjusted to neutrality using 2N HCl. The resulting slurry can be stored at 37° C. for eleven months.

1. customadjusted to 37° C.
2. customwhile purging of the solution of oxygen
3. additionis then added
4. additionThe mixture is then treated with 5 mL's of a hydroxyapatite slurry
5. customThe hydroxyapatite slurry can be prepared
6. dissolutionis dissolved
7. temperature(heating for several days may be necessary)
8. temperatureOnce again the solution is heated
9. customis removed
10. addition1.5 L of distilled water is added
11. waitafter settling again for 2 days
12. customthe supernatant is removed
13. waitThe resulting slurry can be stored at 37° C. for eleven months

ORD record

ord-85b8f75242994a83a1b336b69db37d20

Source dataset

uspto-grants-2006_07

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US07074752B2