反应详情

A solution of (3-{(2R)-2-[(2R)-2-{[tert-butyl(dimethyl)silyl]oxy}-2-(4-hydroxy-3-hydroxymethyl-phenyl)-ethylamino]-propyl}-phenyl)-acetic acid (Preparation 20) (250 mg, 0.45 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (94 mg, 0.49 mmol), hydroxybenzotriazole monohydrate (66 mg, 0.49 mmol) in N,N-dimethylformamide (4 ml) was treated with triethylamine (0.12 ml, 0.89 mmol) and cycloheptylamine (56 mg, 0.49 mmol) and the resulting suspension left to stir at room temperature under a nitrogen atmosphere for 18 hours. The solvent was removed in vacuo and the residue partitioned between ethyl acetate (10 ml) and saturated aqueous sodium bicarbonate (10 ml). The organic phase was separated, and the aqueous phase extracted with further ethyl acetate (2×10 ml). The combined organic extracts were washed with water (5 ml), brine (5 ml), dried (sodium sulfate) and the solvent removed in vacuo. The residue was purified by flash column chromatography on silica gel eluting with dichloromethane:methanol:880 ammonia (95:5:0.5 by volume) to give the title compound as a pale yellow oil (150 mg). 1H NMR (400 MHz, CD3OD): δ=7.20-6.98 (6H, m), 6.68 (1H, d), 4.60 (3H, m), 3.80 (1H, m), 3.40 (2H, s), 2.85 (2H, m), 2.63 (2H, m), 2.58 (1H, m), 1.80 (2H, m), 1.75-1.40 (10H, m), 1.03 (3H, d), 0.83 (9H, s), 0.00 (3H, s), −0.20 (3H, s) ppm. LRMS (electrospray): m/z [M+H]+ 569.

1. waitthe resulting suspension left
2. customThe solvent was removed in vacuo
3. customthe residue partitioned between ethyl acetate (10 ml) and saturated aqueous sodium bicarbonate (10 ml)
4. customThe organic phase was separated
5. extractionthe aqueous phase extracted with further ethyl acetate (2×10 ml)
6. washThe combined organic extracts were washed with water (5 ml), brine (5 ml)
7. dry with materialdried (sodium sulfate)
8. customthe solvent removed in vacuo
9. customThe residue was purified by flash column chromatography on silica gel eluting with dichloromethane