Urea → 2-Hydroxyethyl Methacrylate

HRID 1870690Notes

Reaction equation

HRID 1870690 reaction equation
C(O)[C@H]1O[C@H]([O:8][C@:9]2(CO)[O:13][C@H:12]([CH2:14][OH:15])[C@@H:11](O)[C@@H:10]2O)[C@H](O)[C@@H](O)[C@@H]1O.N[C:25](N)=O.[Cl-].[K+].[O-]S([O-])(=O)=O.[Mg+2].OP([O-])(O)=O.[K+].C(O)[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O>[O-]S([O-])(=O)=O.[Cu+2].[O-]S([O-])(=O)=O.[Mn+2].O.[O-]S([O-])(=O)=O.[Zn+2].O>[CH3:25][C:10]([C:9]([O:13][CH2:12][CH2:14][OH:15])=[O:8])=[CH2:11]

Participants

11

Reactant 6

HCID 1176UreaHCID 4873Potassium ChlorideHCID 5988SucroseHCID 6255beta-MaltoseHCID 24083Sulfuric acid magnesium salt (1:1)HCID 516951Monobasic potassium phosphate

Product 1

HCID 133602-Hydroxyethyl Methacrylate

Reagents, catalysts & solvents 4

HCID 24424Zinc SulfateCatalystHCID 24462Copper SulfateCatalystHCID 177577Manganese sulfate monohydrateCatalystHCID 962WaterSolvent2×

Procedure

Aspergillus oryzae strain SE01-28 described in Example 9 was transformed with pSE38 to create new transformants designated Aspergillus oryzae SE27 according to the method described in Example 8 with the exceptions that Basta resistance was used for selection and 2-8 μg of NdeI digested pSE38 was added per transformation directly from the reaction so that ~5 U of enzyme was included in the transformation mixture. Media for Basta selection contained 20 ml of COVE salts, 1 M sucrose, 25 g/L Noble agar, 10 mM Urea, and either 5 mg/ml Basta (Hoechst Schering, Rodovre, Denmark) for transformation or 10 mg/ml Basta for maintaining transformants. The COVE salts were composed of 26 g of KCl, 26 g of MgSO4 -7H2O, 76 g of KH2PO4, and 50 ml of COVE trace elements per liter of deionized water. The COVE trace elements were composed of 0.04 g of Na2B4O7 -10H2O, 0.4 g of CuSO4 -5H2O, 1.2 g of FeSO4 -7H2O, 0.7 g of MnSO4 -H2O, 0.8 g of Na2MoO2 -H2O, 10 g of ZnSO4 -7H2O per liter of deionized water. Maltose was added to a final concentration of 2.5%, when indicated. Basta selection overlay medium was the same as above except that Basta was added prior to use.

Workup

  1. additionwas added per transformation directly from the reaction so that ~5 U of enzyme
  2. temperatureeither 5 mg/ml Basta (Hoechst Schering, Rodovre, Denmark) for transformation or 10 mg/ml Basta for maintaining transformants
  3. additionwas added

Sources & evidence

ORD record
ord-65b37398084c41e7bd610901b96e0053
Source dataset
uspto-grants-2000_08
Patent number
US06100057

Reaction notes

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