Benzoic Acid → HCID 86759486, compound
Reaction equation
C[O:2][CH:3]([O:13]C)[CH:4]=[C:5](C)[CH2:6][CH2:7][CH:8]=C(C)C.C(O)(=O)C=CC1C=CC=CC=1.C(O)(=O)/C=C/C=C/C.[C:34]([OH:42])(=[O:41])[C:35]1[CH:40]=[CH:39][CH:38]=[CH:37][CH:36]=1.O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O>>[C:3]([OH:13])(=[O:2])/[CH:4]=[CH:5]/[CH:6]=[CH:7]/[CH3:8].[C:34]([OH:42])(=[O:41])[C:35]1[CH:40]=[CH:39][CH:38]=[CH:37][CH:36]=1Participants
Procedure
FIG. 24 shows the combined effect of citral dimethyl acetal, cinnamic acid, sorbic acid and benzoic acid on growth of yeast Saccharomyces cerevisiae X2180-1B in a matrix of tubes of synthetic soft drink, 0% tea. Synthetic soft drink contained glucose, 8%w/v, citric acid 3 g/l, potassium orthophosphate 1 g/l, magnesium chloride 0.1 g/l and yeast extract 0.1 g/l. The matrix of 30 ml tubes each containing 10 ml synthetic soft drink pH 3.4, all contained 100 ppm citral dimethyl acetal. Sorbic acid+Benzoic acid 1:1 ratio, were used in the range 1-250 ppm (e.g. 250 ppm=125 ppm sorbic acid+125 ppm benzoic acid) and cinnamic acid in the range 1-175 ppm. Tubes were inoculated with 104 cells of the yeast Saccharomyces cerevisiae X2180-1B. Tubes were incubated for 14 days at 25° C. to allow surviving yeasts to grow out. At 14 days growth was measured by optical density at 600 nm in x11 diluted samples, and blank values subtracted.
Workup
- extraction8%w/v, citric acid 3 g/l, potassium orthophosphate 1 g/l, magnesium chloride 0.1 g/l and yeast extract 0.1 g/l
- waitAt 14 days growth was measured by optical density at 600 nm
- additionin x11 diluted samples, and blank values
Sources & evidence
- ORD record
- ord-b85fa476fb0a430ca8716c9180de018d
- Source dataset
- uspto-grants-2004_07
- Patent number
- US06761919B2
Reaction notes
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