Sucrose → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

The activity of the present β-fructofuranosidase is assayed as follows: Sucrose as a substrate was dissolved in 20 mM acetate buffer (pH 6.0) containing 5 mM calcium chloride to give a concentration of 0.1 w/v %. To 5 ml of the solution was added 0.2 ml of a test sample, and the mixture was incubated at 40° C. for 10 min, followed by suspending the enzymatic reaction by the addition of 0.2 ml of the Somogyi copper solution and assaying the reducing power of the reaction mixture by the Somogyi-Nelson's method. As a control, a control solution, which was preheated at 100° C. for 10 min to inactivate the enzyme, was treated similarly as above. Under the above reaction conditions, one unit β-fructofuranosidase activity is defined as the amount of enzyme or polypeptide which gives a reducing power equal to that of 2μ mole of glucose per minute.

1. customto give a concentration of 0.1 w/v %
2. customthe enzymatic reaction
3. waitAs a control, a control solution, which was preheated at 100° C. for 10 min
4. additionwas treated similarly as above
5. customUnder the above reaction conditions, one unit β-fructofuranosidase activity

ORD record

ord-13ad195b75d54803934063719f7221a5

Source dataset

uspto-grants-2002_05

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US06383769B1