反应详情

To a solution of 390 mg 5-[2-((S)-2-cyclobutylcarbamoyl-pyrrolidin-1-yl)-2-oxo-ethoxy]-1-phenyl-1H-pyrazole-3-carboxylic acid in 5 ml DMF were added 359 mg HATU and 156 μl DIPEA. After 10 minutes a solution of 326 mg 4-((S)-2-amino-3-tert-butoxycarbonyl-amino-propionyl)-piperazine-1-carboxylic acid ethyl ester in 2 ml DMF was added and after 30 minutes the reaction mixture was diluted with ethyl acetate and extracted with aqueous LiCl (4%), 0.1 M HCl and saturated NaHCO3. The crude product (600 mg) thus obtained after evaporation of the solvent was deprotected in the following step.

1. extractionextracted with aqueous LiCl (4%), 0.1 M HCl and saturated NaHCO3