反应详情

To a solution of 100 mg 5-[2-((S)-2-Cyclobutylcarbamoyl-pyrrolidin-1-yl)-2-oxo-ethoxy]-1-phenyl-1H-pyrazole-3-carboxylic acid in 3 ml DMF were added 92 mg HATU, 82 l DIPEA and 75 mg 4-((S)-2-Amino-4,4,4-trifluoro-butyryl)-piperazine-1-carboxylic acid propyl ester. After stirring for 3 h saturated NaHCO3 solution (1.5 ml) was added and the mixture passed through a Chem Elute® cartridge eluting with DCM. The crude product obtained after evaporation of the solvent was purified by preparative HPLC (C18 reverse phase column, elution with a water/MeCN gradient with 0.1% TFA) to give the title compound.

1. additionwas added
2. washElute® cartridge
3. washeluting with DCM
4. customThe crude product obtained
5. customafter evaporation of the solvent
6. customwas purified by preparative HPLC (
7. washC18 reverse phase column, elution with a water/MeCN gradient with 0.1% TFA)