反应详情

Dissolve 2.5 g. of 5-epigentamicin C1 in 250 ml. of water and add 100 ml. of methanol. Add 0.35 g. of triethylamine and stir for fifteen minutes. Add a solution of 1.0 g. of N-(5-acetoxypentanoyloxy)succinimide with stirring to the solution of the antibiotic, and stir at ambient temperature for 16 hours. Evaporate the solution in vacuo to leave a solid residue. Dissolve the residue in 5 ml. of 5% ethanolic hydrazine hydrate and heat under reflux for 15 minutes. Concentrate the solution in vacuo to leave an oily residue and chromatograph it on 200 g. silica gel in the lower phase of a solvent system consisting of chloroform:methanol:7% ammonium hydroxide (2:1:1) to give 1-N-(5-hydroxypentanoyl)-5-epigentamicin C1.

1. dissolutionDissolve 2.5 g
2. additionAdd 0.35 g
3. additionAdd a solution of 1.0 g
4. customEvaporate the solution in vacuo
5. customto leave a solid residue
6. dissolutionDissolve the residue in 5 ml
7. temperatureunder reflux for 15 minutes
8. concentrationConcentrate the solution in vacuo
9. customto leave an oily residue and chromatograph it on 200 g