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Solid carbontetrabromide (429 mg, 1.29 mmol) was added to a mixture of (8-methyl-2-o-tolylquinolin-3-yl)methanol (227 mg, 0.86 mmol) and triphenyl-phosphine (339 mg, 1.29 mmol) in CH2Cl2 (5 mL) at 0° C., and the mixture was stirred at 0° C. for 0.5 h. The crude mixture was concentrated under reduced pressure, evaporated onto silica gel, and purified by flash chromatography (Biotage® Si 25+M) eluting with EtOAc/hexane (0% to 10%) to provide an off-white solid; used without further purification, MS (ESI+) m/z=326.0 (M). A 2.0M aqueous solution of sodium hydroxide (0.86 mL, 1.72 mmol) was added to a mixture of 3-(bromomethyl)-8-methyl-2-o-tolylquinoline (140 mg, 0.43 mmol) and 6-mercaptopurine monohydrate (146 mg, 0.86 mmol) in THF (1.6 mL), and the biphasic mixture was heated under reflux for 5 h. The reaction mixture was cooled to 0° C., neutralized by the addition of 1N aqueous HCl, diluted with brine (10 mL), and extracted with THF (3×15 mL). The combined organic layers were dried (MgSO4) and concentrated under reduced pressure. The resulting yellow solid was dissolved in THF/DMSO, evaporated onto silica gel, and purified by flash chromatography (Biotage® Si 25+M) eluting with acetone/hexane (20% to 50%). The resulting off-white solid was recrystallized from THF/MeOH to provide a white solid [PI3Kδ IC50=889 nM]. MS (ESI+) m/z=398.1 (M+1).

1. concentrationThe crude mixture was concentrated under reduced pressure
2. customevaporated onto silica gel
3. custompurified by flash chromatography (Biotage® Si 25+M)
4. washeluting with EtOAc/hexane (0% to 10%)
5. customto provide an off-white solid
6. customused without further purification, MS (ESI+) m/z=326.0 (M)
7. temperaturethe biphasic mixture was heated
8. temperatureunder reflux for 5 h
9. temperatureThe reaction mixture was cooled to 0° C.
10. extractionextracted with THF (3×15 mL)
11. dry with materialThe combined organic layers were dried (MgSO4)
12. concentrationconcentrated under reduced pressure
13. dissolutionThe resulting yellow solid was dissolved in THF/DMSO
14. customevaporated onto silica gel
15. custompurified by flash chromatography (Biotage® Si 25+M)
16. washeluting with acetone/hexane (20% to 50%)
17. customThe resulting off-white solid was recrystallized from THF/MeOH
18. customto provide a white solid [PI3Kδ IC50=889 nM]