Potassium Chloride → Dopamine

HRID 2061631Notes

Reaction equation

HRID 2061631 reaction equation
C1C2(N(C3C=CC=CC=3)CNC2=[O:8])CCN(CCCC(C2C=CC(F)=CC=2)=O)C1.CCC[N:33]1[C@H:38]2CO[C:41]3[CH:46]=[CH:45][C:44]([OH:47])=[CH:43][C:42]=3[C@@H:37]2OCC1.C(O)C(N)(CO)CO.Cl.[Cl-].[K+].[Mg+2].[Cl-].[Cl-].[Cl-].[Cl-].[Ca+2].CC([C@]1(O)C[C@H]2N(C[C@H]3C4C2=CC=CC=4CCC2C=CC=CC3=2)CC1)(C)C>>[NH2:33][CH2:38][CH2:37][C:42]1[CH:41]=[CH:46][C:45]([OH:8])=[C:44]([OH:47])[CH:43]=1

Participants

8

Reactant 7

HCID 4873Potassium ChlorideHCID 5265Spiperone4×HCID 37461ButaclamolHCID 93573Tromethamine hydrochlorideHCID 115107rel-(4aR,10bR)-3,4,4a,10b-Tetrahydro-4-propyl-2H,5H-[1]benzopyrano[4,3-b]-1,4-oxazin-9-olHCID 5284359Calcium ChlorideHCID 5360315Magnesium Chloride

Product 1

HCID 681Dopamine

Procedure

[3H]Spiperone binding assays. [3H]spiperone binding assays for D2-like receptors were performed as previously described in detail (29,31) and as described for [3H]PD 128907 except for the following. Assays were performed using membranes prepared from rat caudate-putamen, which predominantly express the D2 subtype of D2-like receptors, and the final membrane homogenate concentration was 1.5 mg o.w.w./ml. The assay buffer was 50 mM Tris-HCl, 5 mM KCl, 2 mM MgCl2, and 2 mM CaCl2, pH 7.4 at 23° C.; the concentration of [3H]spiperone (24 Ci/mmol; Amersham) was 0.2 nM; and the incubation time was 90 minutes at 23° C. Nonspecific binding was defined in the presence of 1 μM (+)-butaclamol. Ki values were determined using the KD value for [3H]spiperone of 0.1 nM (29).

Workup

  1. customprepared from rat caudate-putamen, which
  2. customat 23° C.

Sources & evidence

ORD record
ord-95e89d994ef74c6f892edfeb251b6df8
Source dataset
uspto-grants-2012_10
Patent number
US08283368B2

Reaction notes

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