反应详情

The compound (203 mg) obtained in Example 1-2 was dissolved in DMF (5.0 ml) and chloroform (5.0 ml), and then added with triethylamine (0.374 ml), WSCI hydrochloride (382 mg), HOBt (200 mg), and the compound (463 mg) obtained in Example 6-1. The whole was stirred at room temperature for 23 hours. After completion of the reaction, the solvent was distilled off. Then, the resultant was added with chloroform and washed with water and a saturated saline solution, followed by drying with anhydrous sodium sulfate. The solvent was distilled off and the residue was purified through silica gel column chromatography (chloroform/methanol/water), thereby obtaining the subject compound (168 mg) as colorless foam.

1. customAfter completion of the reaction
2. distillationthe solvent was distilled off
3. additionThen, the resultant was added with chloroform
4. washwashed with water
5. dry with materialby drying with anhydrous sodium sulfate
6. distillationThe solvent was distilled off
7. customthe residue was purified through silica gel column chromatography (chloroform/methanol/water)