L-Leucine → L-Isoleucine

HRID 2169116Notes

Reaction equation

HRID 2169116 reaction equation
O=[CH:2][C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.S([O-])([O-])(=O)=O.[NH4+].[NH4+].P([O-])(O)(O)=O.[K+].P([O-])([O-])(O)=O.[K+].[K+].C(=O)([O-])[O-].[Ca+2].S([O-])([O-])(=O)=O.[Mg+2].[NH2:44][C@H:45]([C:50]([OH:52])=[O:51])[CH2:46][CH:47]([CH3:49])C>S([O-])([O-])(=O)=O.[Fe+2].S([O-])([O-])(=O)=O.[Mn+2].S([O-])([O-])(=O)=O.[Zn+2].S([O-])([O-])(=O)=O.[Cu+2]>[NH2:44][C@H:45]([C:50]([OH:52])=[O:51])[C@H:46]([CH2:47][CH3:49])[CH3:2]

Participants

12

Reactant 7

HCID 6106L-LeucineHCID 10112Calcium CarbonateHCID 24083Sulfuric acid magnesium salt (1:1)HCID 24450Potassium Phosphate, DibasicHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2×HCID 516951Monobasic potassium phosphateHCID 6097028Ammonium Sulfate

Product 1

HCID 6306L-Isoleucine

Reagents, catalysts & solvents 4

HCID 24393Ferrous SulfateCatalystHCID 24424Zinc SulfateCatalystHCID 24462Copper SulfateCatalystHCID 24580Manganese SulfateCatalyst

Conditions

Temperature
33 °C

Procedure

In each case, 10 ml of Caso broth containing 0.5% glucose were inoculated with 100 μl of a bacterial culture from example 10 (ATCC 14310 derivatives) in a conical flask with baffles (volume: 100 ml) and shaken at 200 rpm by way of a preculture at 33° C. for 16 h (amplitude: 5 cm). From the preculture, 10 ml of main culture in each of three 100 ml conical flasks with baffles were inoculated with an inoculation volume of 1%. A medium containing 40 g of glucose, 20 g of ammonium sulphate, 0.5 g of potassium dihydrogen phosphate, 0.5 g of dipotassium hydrogen phosphate, 20 g of calcium carbonate, 0.25 g of magnesium sulphate (heptahydrate), 10 mg of iron sulphate (heptahydrate), 10 mg of manganese sulphate (×4H2O), 1 mg of zinc sulphate (heptahydrate), 0.2 mg of copper sulphate and 300 mg/l of L-leucine was used for the main culture.

Workup

  1. customwere inoculated with 100 μl of a bacterial culture from example 10 (ATCC 14310 derivatives) in a conical flask with baffles (volume: 100 ml)

Sources & evidence

ORD record
ord-5b0bad4eaa3741fc92e6722cd26c113e
Source dataset
uspto-grants-2015_06
Patent number
US09045762B2

Reaction notes

Loading…