L-Phenylalanine → 2-Propenoic acid, 3-phenyl-

First, cells were recovered from the culture by centrifugation. The collected cells were washed by suspending them in a 0.85% sodium chloride solution and recovered by centrifugation. The washed cells were then suspended in a 25 mM Tris-HCl buffer solution (pH 8.8) to give a cell concentration of about 1% by wet weight. The suspension was added to an enzymatic reaction medium comprising a 25 mM Tris-HCl buffer solution (pH 8.8) supplemented with 25 mM L-phenylalanine and 0.005% cetyl pyridium hydrochloride. The resultant reaction medium was incubated at 30° C. for 20 minutes. After the reaction was terminated by addition of 1N HCl, cinnamic acid formed in the reaction mixture was analyzed by liquid chromatography to estimate the PAL activity. One unit (U) as defined herein corresponds to the amount of enzyme to produce one micromole of cinnamic acid per minute.

1. customFirst, cells were recovered from the culture by centrifugation
2. washThe collected cells were washed
3. customrecovered by centrifugation
4. customto give a cell concentration of about 1% by wet weight
5. additionThe suspension was added to an enzymatic reaction medium
6. customThe resultant reaction medium
7. customAfter the reaction was terminated by addition of 1N HCl, cinnamic acid
8. customformed in the reaction mixture

ORD record

ord-a75d3011df5348cbabd60acdc676a574

Source dataset

uspto-grants-1991_08

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05043277