反应详情

To the title compound of Example 223 Step B (0.2 mmol, 85 mg) in 1.6 mL of dioxane was added a solution of LiOH (0.8 mmol, 19 mg) in 0.8 mL of H2O. The reaction was allowed to stir at ambient temperature overnight. The crude reaction mixture was poured into pH 7 buffer/EtOAc, which was acidified with 2 N HCl until two clear layers formed after agitation. The organic phase was collected and the aqueous phase was extracted twice with EtOAc. The combined organic phase was dried over MgSO4, then concentrated under reduced pressure to afford the product as a white foam. MS (ESI): m/z 426 (M+H). HPLC A: 1.79 min.

1. customThe crude reaction mixture
2. customformed after agitation
3. customThe organic phase was collected
4. extractionthe aqueous phase was extracted twice with EtOAc
5. dry with materialThe combined organic phase was dried over MgSO4
6. concentrationconcentrated under reduced pressure