反应详情

1 ml of a buffer (20 MM Tris-HCl buffer, 1 mM EDTA, pH 7.5) containing human blood platelet microsomes (50 μg protein/ml) and the test compound (final concentration 10−7M) was agitated, then incubated at 0° C. for 30 minutes. To this was added prostaglandin H2 (100 ng/2 μl). This was incubated at 23° C. for 3 minutes to cause a reaction. Next, 1M hydrochloric acid was added to make the solution acidic and stop the reaction, then this was neutralized by 1M Tris-Base and centrifuged at 3000 rpm for 20 minutes. The amount of the TxB2 in the supernatant was measured by the EIA method (Cayman Co. kit).

1. waitThis was incubated at 23° C. for 3 minutes
2. customa reaction
3. customthe reaction
4. waitcentrifuged at 3000 rpm for 20 minutes