Hydrochloric Acid → alpha-Maltotriose

HRID 2309790Notes

Reaction equation

HRID 2309790 reaction equation
C([O-])(=O)C.[Ca+2].C([O-])(=O)C.[CH2:10]([OH:32])[C@H:11]1[O:16][C@H:15]([O:17][C@H:18]2[C@H:23]([OH:24])[C@@H:22]([OH:25])[C@H:21]([OH:26])[O:20][C@@H:19]2[CH2:27][OH:28])[C@H:14]([OH:29])[C@@H:13]([OH:30])[C@@H:12]1[OH:31].Cl.[O:34]=[CH:35][C@@H:36]([C@H:38]([C@@H:40]([C@@H:42]([CH2:44][OH:45])[OH:43])O)[OH:39])[OH:37]>C([O-])(=O)C.[Na+]>[CH2:10]([OH:32])[C@H:11]1[O:16][C@H:15]([O:17][C@H:18]2[C@H:23]([OH:24])[C@@H:22]([OH:25])[C@@H:21]([O:26][C@H:40]3[C@H:38]([OH:39])[C@@H:36]([OH:37])[C@@H:35]([OH:34])[O:43][C@@H:42]3[CH2:44][OH:45])[O:20][C@@H:19]2[CH2:27][OH:28])[C@H:14]([OH:29])[C@@H:13]([OH:30])[C@@H:12]1[OH:31]

Participants

6

Reactant 4

HCID 313Hydrochloric AcidHCID 6116Calcium AcetateHCID 6255beta-Maltose2×HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Product 1

HCID 192826alpha-Maltotriose

Reagents, catalysts & solvents 1

HCID 517045Sodium AcetateSolvent

Procedure

Further, each fraction of FG3 and FG5 was taken out and dissolved in a 0.1M sodium acetate buffer containing 10 mM calcium acetate buffer solution (pH 5.5), followed by addition of takaamylase A for hydrolyses at 37° C. for 3 hours. The hydrolyzed products were tested by HPLC and TLC. From FG5, there were produced FG3 and maltose, but FG3 was not hydrolyzed by takaamylase A. When FG3 was added to 0.3N HCl and hydrolyzed at 100° C. for 30 minutes, the products were maltose, glucose and 2 kinds of compounds having fluorescence but no maltotriose was produced.

Workup

  1. additionfollowed by addition of takaamylase A for hydrolyses at 37° C. for 3 hours
  2. customFrom FG5, there were produced FG3

Sources & evidence

ORD record
ord-3223f50f9d39406590b342fc81ee81d5
Source dataset
uspto-grants-1987_09
Patent number
US04697006

Reaction notes

Loading…