4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid → Squalene

HRID 2379452Notes

Reaction equation

C1N(CCO)CCN([CH2:10][CH2:11]S(O)(=O)=O)C1.[F-].[K+].[CH2:18](S)[C@@H:19](O)[C@H:20](O)[CH2:21]S>>[CH3:18][C:19](=[CH:20][CH2:21][CH2:18]/[C:19](=[CH:20]/[CH2:21][CH2:10]/[C:11](=[CH:10]/[CH2:11][CH2:18]/[CH:19]=[C:20](/[CH2:21][CH2:18]/[CH:19]=[C:20](/[CH2:10][CH2:11][CH:10]=[C:10]([CH3:11])[CH3:11])\[CH3:21])\[CH3:21])/[CH3:20])/[CH3:19])[CH3:18]

Participants

Reactant 3

HCID 238314-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid2×

HCID 446094D-1,4-Dithiothreitol

HCID 522689Potassium Fluoride2×

Product 1

HCID 638072Squalene

Procedure

Buffer mixture contains 270 mM HEPES, pH 7.5, 20 mM Potassium fluoride and 5.4 mM Dithiothreitol(DTT). 55 μL of this mixture was used per assay. The final concentrations of HEPES, KF and DTT in the assay are 150 mM, 11 I mM and 3 mM respectively.

Sources & evidence

ORD record: ord-380910e2a2a54b63893a61517ba5347b
Source dataset: uspto-grants-1994_11
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05362752

Reaction notes

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