Sodium hydride → HCID 58486268, compound

DMF (3 mL) was added to a mixture of NaH (60%, 171.5 mg, 4.29 mmol) and trimethylsulfoxonium iodide (246.2 mg, 1.12 mmol). The resulting mixture was stirred at rt for 10 min followed by the addition of 5-methoxy-3-((3-((E)-2-(6-(4-methylpiperazin-1-yl)pyridin-3-yl)vinyl)-1H-indazol-6-yl)methylene)indolin-2-one dihydrochloride (E:Z mixture, 155.5 mg, 0.275 mmol) as a suspension in DMF (9 mL, divided for transfer and vial rinse). The reaction mixture was then heated to 55° C. for 6 h, then stirred at rt for 18 h prior to quenching by addition of water (15 mL) and brine (15 mL). The mixture was extracted with EtOAc (225 mL) and the organic layer was washed with brine (2×15 mL), dried over Na2SO4 and concentrated in vacuo. Co-evaporation of DMF with toluene (2×10 mL) in vacuo gave a viscous oil, which was triturated with hexane (3×5 mL) to give a sticky solid. The crude product was purified by silica gel chromatography (5-7.5% 2M NH3-MeOH in DCM) to yield an 85:15 mixture of the title compound and the (1R*,2R*) diastereomer. The major isomer was isolated by silica gel chromatography (3-5% MeOH and 2% Et3N in CHCl3) to yield a sticky yellow solid which was triturated with Et2O to give the title compound as a yellow powder (57.4 mg, 41%). 1H NMR (400 MHz, CD3OD) δ ppm 8.26 (s, 1H), 8.00 (d, J=8.4 Hz, 1H), 7.92 (d, J=9.2 Hz, 1H), 7.45 (s, 1H), 7.40 (d, J=16.8 Hz, 1H), 7.28 (d, J=16.8 Hz, 1H), 7.03 (d, J=7.6 Hz, 1H), 6.87 (d, J=9.2 Hz, 1H), 6.83 (d, J=8.4 Hz, 1H), 6.61 (dd, J=8.4, 2.4 Hz, 1H), 5.58 (d, J=2.0 Hz, 1H), 3.61 (m, 4H), 3.38 (m, 1H), 3.26 (s, 3H), 2.57 (m, 4H), 2.36 (s, 3H), 2.21 (m, 2H); MS ESI [M+H]+ 507.2, calcd for [C30H30N6O2+H]+ 507.2.

1. temperatureThe reaction mixture was then heated to 55° C. for 6 h
2. stirringstirred at rt for 18 h
3. customto quenching by addition of water (15 mL) and brine (15 mL)
4. extractionThe mixture was extracted with EtOAc (225 mL)
5. washthe organic layer was washed with brine (2×15 mL)
6. dry with materialdried over Na2SO4
7. concentrationconcentrated in vacuo
8. customCo-evaporation of DMF with toluene (2×10 mL) in vacuo
9. customgave a viscous oil, which
10. customwas triturated with hexane (3×5 mL)
11. customto give a sticky solid
12. customThe crude product was purified by silica gel chromatography (5-7.5% 2M NH3-MeOH in DCM)
13. customto yield

ORD record

ord-bf0344e6c47b43328b913b227253cac2

Source dataset

uspto-grants-2012_09

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08263596B2