反应详情

1-Iodopropane (0.1 mmol) was weighed into a vial and an aliquot (0.3 ml, 0.1 mmol) of a solution of 2-(butyloxy)-8-(methyloxy)-9-(3-piperidinylmethyl)-9H-purin-6-amine (802 mg, 2.398 mmol) suspended in DMF (7.2 ml) was added. DIPEA (40 μl, 0.229 mmol) was added and the tube was capped and shaken to aid dispersion then allowed to stand at room temperature (18 hours). An additional portion of 1-iodopropane (0.05 mmol) was added and the reaction allowed to stand for 18 hours. DMSO (400 uL) was added and the mixture purified by Mass Directed AutoPrep (Method A). The solvent was evaporated in vacuo using the Genevac. The residue was redissolved in 4M HCl/dioxane (0.5 ml), capped and stood at room temperature for 18 hours. The solvent was dried under a stream of nitrogen in the Radleys blowdown apparatus. The material was redissolved in methanol (0.5 ml), and applied to a 0.5 g aminopropyl SPE (preconditioned with methanol, 2 CV). The cartridge was eluted with methanol (2 ml), the solvent was removed to give the title compound (7.5 mg).

1. additionwas added
2. customthe tube was capped
3. customto stand at room temperature
4. custom(18 hours)
5. customthe mixture purified by Mass Directed AutoPrep (Method A)
6. customThe solvent was evaporated in vacuo
7. dissolutionThe residue was redissolved in 4M HCl/dioxane (0.5 ml)
8. customcapped
9. waitstood at room temperature for 18 hours
10. customThe solvent was dried under a stream of nitrogen in the Radleys blowdown apparatus
11. dissolutionThe material was redissolved in methanol (0.5 ml)
12. washThe cartridge was eluted with methanol (2 ml)
13. customthe solvent was removed