Hydrochloric Acid → HCID 57388939, compound

Temperature

60 °C

To a solution of methyl 6-(2-cyclopropyl-1-(4′-(trifluoromethyl)biphenyl-4-yl)ethylamino)nicotinate (60 mg, 0.14 mmol) in THF (3.0 mL) was added 170 μL of a 2M aqueous solution of sodium hydroxide (0.34 mL). The reaction solution was stirred at 60° C. overnight. The mixture was acidified to pH 3 by addition of 1N aqueous HCl. The mixture was extracted with ethyl acetate (5 mL×3). The combined organic layers were washed with water, brine, then dried over Na2SO4 and concentrated under reduced pressure. The residue was dissolved in DMF (3 mL). methyl 3-aminopropionate hydrochloride (32.7 mg, 0.234 mmol) was added. HATU (134 mg, 0.351 mmol) was added. The mixture was stirred for 15 mins. Diisopropylethylamine (60.5 mg, 0.468 mmol) was added. The resulting mixture was stirred 2 h at room temperature. The reaction mixture was poured into water and the phases separated. The aqueous layer was extracted with ethyl acetate (3 mL×3). The combined organic layers were washed with water, dried over Na2SO4 and concentrated under reduced pressure to give a yellow solid. This solid was dissolved in THF (1.5 mL). A solution of lithium hydroxide dihydrate (14.7 mg, 0.351 mmol) in water (1.5 mL) was added. The reaction mixture was stirred for 1 h at room temperature. The mixture was acidified to pH 3 by addition of 1N aqueous HCl. The mixture was extracted with ethyl acetate (5 mL*3), and the combined organic layers were washed with water, brine, dried over Na2SO4 and concentrated to dryness. Purification by HPLC (column: Kromasil Eternity-5-C18 150×30 mm×5 μm; modifier 0.0685% TFA; gradient 10 to 80% acetonitrile in water) gave 3-(6-(2-cyclopropyl-1-(4′-(trifluoromethyl)biphenyl-4-yl)ethylamino)nicotinamido)propanoic acid (7.00 mg) as a colorless solid. 1H NMR (400 MHz, Methanol-d4) δ 8.32 (s, 1H), 7.72-7.67 (m, 3H), 7.60 (d, J=8.4 Hz, 2H), 7.53 (d, J=8.4 Hz, 2H), 7.40 (d, J=8.4 Hz, 2H), 6.43 (d, J=8.8 Hz, 1H), 4.90-5.00 (m, 1H), 3.40-3.48 (m, 2H), 2.40-2.45 (m, 2H), 1.83-1.74 (m, 1H), 1.65-1.55 (m, 1H), 0.75-0.60 (m, 1H), 0.45-0.28 (m, 2H), 0.18-0.00 (m, 2H). MS (M+1)=498.3.

1. extractionThe mixture was extracted with ethyl acetate (5 mL×3)
2. washThe combined organic layers were washed with water, brine
3. dry with materialdried over Na2SO4
4. concentrationconcentrated under reduced pressure
5. dissolutionThe residue was dissolved in DMF (3 mL)
6. stirringThe mixture was stirred for 15 mins
7. stirringThe resulting mixture was stirred 2 h at room temperature
8. customthe phases separated
9. extractionThe aqueous layer was extracted with ethyl acetate (3 mL×3)
10. washThe combined organic layers were washed with water
11. dry with materialdried over Na2SO4
12. concentrationconcentrated under reduced pressure
13. customto give a yellow solid
14. stirringThe reaction mixture was stirred for 1 h at room temperature
15. extractionThe mixture was extracted with ethyl acetate (5 mL*3)
16. washthe combined organic layers were washed with water, brine
17. dry with materialdried over Na2SO4
18. concentrationconcentrated to dryness
19. customPurification by HPLC (column: Kromasil Eternity-5-C18 150×30 mm×5 μm; modifier 0.0685% TFA; gradient 10 to 80% acetonitrile in water)

ORD record

ord-44e19ead96fb4a18b504364fdaded73f

Source dataset

uspto-grants-2014_08

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08809342B2