反应详情

温度

-20 °C

2.493 g of Boc-D-Leu-OH.H2O was dissolved in 10 ml of dimethylformamide. The solution was cooled to -20° C. 1.1 ml of N-methylmorpholine and 0.96 ml of ethyl chlorocarbonate were added to the solution. After 5 min, a solution of 4.414 g of CF3COOH.H-Arg(Tos)-NH2 and 1.65 ml of N-methylmorpholine in 20 ml of dimethylformamide was added thereto and the mixture was stirred at about -5° C. for 2 h. After concentration, the residue was dissolved in ethyl acetate and washed with NaHCO3 and water successively. After concentration, ether was added thereto to solidify the product. Thus, 4.96 g of Boc-D-Leu-Arg(Tos)-NH2 was obtained.

1. concentrationAfter concentration
2. dissolutionthe residue was dissolved in ethyl acetate
3. washwashed with NaHCO3 and water successively
4. concentrationAfter concentration, ether
5. additionwas added