反应详情

Each of the strain BPL-31 and its parent strain FERM BP-1777 was cultured at 30° C. for 24 hours on the BYG agar medium and then inoculated into a test tube charged with 6 ml of a seed medium (a medium prepared by dissolving 20 g of glucose, 15 g of peptone, 15 g of yeast extract, 2.5 g of sodium chloride, 1 g of urea, 200 mg of L-phenylalanine and 200 mg of L-tyrosine in 1 liter of water and adjusting its pH to 7.2) and cultured at 30° C. for 24 hours. Next, 2 ml of the seed culture was inoculated into a 300 ml capacity conical flask charged with 20 ml of a main culture (a medium prepared by dissolving 60 g of glucose, 10 g of corn steep liquor, 20 g of ammonium sulfate, 0.5 g of dipotassium hydrogenphosphate, 0.5 g of potassium dihydrogenphosphate, 0.25 g of magnesium sulfate heptahydrate, 10 mg of manganese sulfate heptahydrate and 0.03 g of biotin in 1 liter of water, adjusting its pH to 7.2 and then adding 20 g of calcium carbonate thereto) and cultured at 30° C. for 72 hours.

1. custominoculated into a test tube
2. additioncharged with 6 ml of a seed medium (a medium
3. customprepared
4. extractionextract
5. additioncharged with 20 ml of a main culture (a medium
6. customprepared
7. wait) and cultured at 30° C. for 72 hours