反应详情

2.0 g (8.96 mmol) of 7-nitro-1-benzothiophene-2-carboxylic acid and 1.78 g (8.96 mmol) of R-3-aminoquinuclidine dihydrochloride are initially charged in 25 ml of DMF. At 0° C., 2.06 g (10.75 mmol) of EDC, 1.45 g (10.75 mmol) of HOBt and 4.50 ml (32.26 mmol) of triethylamine are added to the solution. The mixture is stirred at RT for 18 h. The reaction is terminated by addition of a 10% strength aqueous sodium bicarbonate solution, and ethyl acetate is added. The resulting precipitate is filtered off with suction. The mother liquor is extracted twice with ethyl acetate. The organic phases are combined and concentrated. Both the precipitate and the concentrated mother liquor are purified by silica gel column chromatography (mobile phase: dichloromethane/methanol/25% ammonia 100:10:2). The product fractions are concentrated, dissolved in a mixture of methanol and 1N hydrochloric acid, then again concentrated and dried under high vacuum. The resulting solid is triturated with acetonitrile, filtered off with suction and dried at 50° C. under reduced pressure. 1.10 g (32.8% of theory) of the title compound are obtained.

1. customThe reaction is terminated by addition of a 10% strength aqueous sodium bicarbonate solution, and ethyl acetate
2. additionis added
3. filtrationThe resulting precipitate is filtered off with suction
4. extractionThe mother liquor is extracted twice with ethyl acetate
5. concentrationconcentrated
6. customBoth the precipitate and the concentrated mother liquor are purified by silica gel column chromatography (mobile phase: dichloromethane/methanol/25% ammonia 100:10:2)
7. concentrationThe product fractions are concentrated
8. dissolutiondissolved in a mixture of methanol and 1N hydrochloric acid
9. concentrationagain concentrated
10. customdried under high vacuum
11. customThe resulting solid is triturated with acetonitrile
12. filtrationfiltered off with suction
13. customdried at 50° C. under reduced pressure