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HRID405668

反应详情

EQUATION

反应方程式

HRID 405668 的结构方程式

REACTANTS

反应物

6
结构：C=C(OP(=O)(O)O)C(=O)O
HCID1005

Phosphoenolpyruvic acid

C3H5O6P

结构：[Cl-].[K+]
HCID4873

Potassium chloride

ClK

结构：Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
HCID5957

Adenosine triphosphate

C10H16N5O13P3

结构：CC(=O)C(=O)[O-]
HCID107735

Pyruvate

C3H3O3-

结构：[Cl-].[Cl-].[Mg+2]
HCID5360315

Magnesium Chloride

Cl2Mg

结构：O=C1O[C@H]([C@@H](O)CO)C(O)=C1O
HCID54670067

L-Ascorbic acid

C6H8O6

PRODUCTS

生成物

1
结构：O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
HCID107526

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

1
结构：O=S(=O)(O)CCN1CCOCC1
溶剂HCID78165

4-Morpholineethanesulfonic acid

C6H13NO4S

PROCEDURE

实验过程

In 10.0 mM 2-morpholinoethanesulfonic acid (MES) buffer, 17.6 mM MgCl2, 17.6 mM KCl, 175.7 mM phosphoenolpyruvic acid (PEP), 17.6 mM ATP, 123 U/mL pyruvate kinase (PK), 97 U/mL hexokinase, and 20 U/mL ascorbic acid oxidase were dissolved in this composition, and the pH was adjusted to 7.0 to obtain a glucose converting reagent.