反应详情

To a solution of 0.035 mole of t-butyloxycarbonylalanine in 80 ml of dry tetrahydrofuran which has been cooled to -20° to - 30° is added 4 ml of N-methylmorpholine and then 4.6 ml of isobutylchloroformate. After stirring for 10 minutes there is added 0.035 mole of alanylproline benzylester hydrochloride and 4 ml of N-methylmorpholine in 60 ml of chloroform. The reaction mixture is stirred overnight at room temperature, concentrated in vacuo and the residue taken up between ethyl acetate and 0.25 N hydrochloric acid. The organic layer is separated, washed with saturated sodium bicarbonate, water, dried over magnesium sulfate and concentrated in vacuo to give t-butoxycarbonylalanylalanylproline benzyl ester.

1. stirringThe reaction mixture is stirred overnight at room temperature
2. concentrationconcentrated in vacuo
3. customThe organic layer is separated
4. washwashed with saturated sodium bicarbonate, water
5. dry with materialdried over magnesium sulfate
6. concentrationconcentrated in vacuo