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HRID438959

反应详情

EQUATION

反应方程式

HRID 438959 的结构方程式

REACTANTS

反应物

2
结构：CN[C@@H]1C[C@H]2O[C@@](C)([C@@H]1OC)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4
HCID44259

Staurosporine

C28H26N4O3

结构：O=Cc1ccc(-c2nn[nH]n2)cc1
HCID2760606

4-(2H-tetrazol-5-yl)benzaldehyde

C8H6N4O

PRODUCTS

生成物

3
结构：CN[C@@H]1C[C@H]2O[C@@](C)([C@@H]1OC)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4
HCID44259

Staurosporine

C28H26N4O3

结构：O=Cc1ccc(-c2nn[nH]n2)cc1
HCID2760606

4-(2H-tetrazol-5-yl)benzaldehyde

C8H6N4O

结构：CO[C@@H]1[C@H](N(C)Cc2ccc(-c3nn[nH]n3)cc2)C[C@H]2O[C@]1(C)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4
HCID178496673

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

4
结构：CC(=O)O
试剂HCID176

Acetic Acid

C2H4O2

结构：CC(C)[O-].CC(C)[O-].CC(C)[O-].CC(C)[O-].[Ti+4]
试剂HCID11026

2-Propanol, titanium(4+) salt

C12H28O4Ti

结构：CC(=O)O[BH-](OC(C)=O)OC(C)=O.[Na+]
试剂HCID5049666

Sodium triacetoxyborohydride

C6H10BNaO6

结构：CN1CCCC1=O
溶剂HCID13387

N-Methyl-2-pyrrolidone

C5H9NO

7 次

CONDITIONS

反应条件

温度
22 °C

WORKUP

后处理

  1. additionThe next morning, the Mosquito(R) was used to dilute the 100 mM reactions to 50 mM via addition of 1 uL DMSO to each reaction well, followed by dispense mixing.
  2. additionThe reactions were quenched with 1 uL saturated aqueous NaHCO3.
  3. additionThe reactions were quenched with 1 uL water.
  4. additionThe reactions were diluted to approximately 10 mM.
  5. aliquotMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.
  6. additionMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.