反应详情

5.0 gr of sphingosine (16.70) mM), prepared according to Example 4, are reacted with 2.1 gr of the sodium salt of fluoroacetic acid (20.88 mM), in the presence of 5.3 gr of 2-chloro-1-methylpyridine iodide (20.88 mM) and 5.8 cc of triethylamine (42.00 mM) in 80 cc of dimethylformamide, at room temperature overnight. The solvent is evaporated, and the residue is gathered in CH2Cl2 and washed with H2O. It is anhydrified and purified by chromatography, eluting with a mixture of CH2Cl2 /ethyl acetate/CH3CH 70:30:1 v/v/v.

1. customThe solvent is evaporated
2. washwashed with H2O
3. custompurified by chromatography
4. washeluting with a mixture of CH2Cl2 /ethyl acetate/CH3CH 70:30:1 v/v/v