反应详情

温度

-15 °C

Dibenzylphosphoryl-L-alanyl-L-proline (1.4 g, 3.2 m mole), HOBt (476 mg, 3.5 m mole) and oily D-proline benzylester hydrochloride (968 mg, 4 m mole) were dissolved in DMF (5 ml) and WSC (0.64 ml, 3.5 m mole) were added thereto while cooling to -15° C. The reaction was carried out for 3 hours under cooling and then overnight at room temperature, and ethyl acetate (100 ml) was added to the reaction solution. The mixture was washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order, and then dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain an oily dibenzylphosphoryl-L-alanyl-L-prolyl-D-proline benzylester.

1. temperatureunder cooling
2. washThe mixture was washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order
3. dry with materialdried with anhydrous sodium sulfate
4. distillationThe solvent was distilled off under reduced pressure