反应详情

H-L-Phe(4-NO2)—NMe (200 mg, 0.770 mmol) is dissolved in 1 mL DMF. Diisopropylethylamine (209 mg, 1.62 mmol), EDC (162 mg, 0.847 mmol), HOBt.H2O (130 mg, 0.847 mmol), and Boc-Tyr (238 mg, 0.847 mmol) are added and the mixture is stirred for 18 hr. at 20° C. The mixture is partitioned between water and EtOAc (2×60 mL). The organics are combined and washed with brine, dried over MgSO4, filtered and evaporated to give crude product. Purification by flash chromatography, which is eluted with 97:3 DCM:methanol to give pure white solid.

1. customat 20° C
2. customThe mixture is partitioned between water and EtOAc (2×60 mL)
3. washwashed with brine
4. dry with materialdried over MgSO4
5. filtrationfiltered
6. customevaporated
7. customto give crude product
8. customPurification by flash chromatography, which
9. washis eluted with 97:3 DCM
10. custommethanol to give pure white solid