反应详情

Cells of G. oxydans DSM 17078 were grown at 27° C. for 3 days on No. 3BD agar medium containing 70 g/l L-sorbose, 0.5 g/l glycerol, 7.5 g/l yeast extract (Difco), 2.5 g/l MgSO4.7H2O, 10 g/l CaCO3 and 18 g/l agar (Difco). The resting cell reactions (1 ml reaction mixture in 10 ml tube) were carried out with 2% D-sorbitol, 2% L-sorbose, or 1% L-sorbosone at 30° C. for 24 h as described in Example 2. Strain DSM 17078 produced 280, 400 and 1780 mg/l of Vitamin C from D-sorbitol, L-sorbose, and L-sorbosone, respectively.

1. extractionextract (Difco), 2.5 g/l MgSO4.7H2O, 10 g/l CaCO3 and 18 g/l agar (Difco)
2. customThe resting cell reactions (1 ml reaction mixture in 10 ml tube)