huagongshe.com
HRID532103

反应详情

EQUATION

反应方程式

HRID 532103 的结构方程式

REACTANTS

反应物

9
结构：CC(=O)[O-]
HCID175

Acetate

C2H3O2-

结构：CC(=O)C(=O)O
HCID1060

Pyruvic acid

C3H4O3

结构：Nc1ccn([C@@H]2O[C@H](COP(=O)(O)O)[C@@H](O)[C@H]2O)c(=O)n1
HCID6131

Cytidine 5′-monophosphate

C9H14N3O8P

2 次

结构：[Na+].[OH-]
HCID14798

Sodium Hydroxide

HNaO

结构：O.[Cl-].[Cl-].[Mg+2]
HCID168060

未命名化合物

结构：CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O
HCID439174

N-Acetylglucosamine cyclized form

C8H15NO6

结构：CC(=O)N[C@H]1[C@H]([C@H](O)[C@H](O)CO)OC(O)(C(=O)O)C[C@@H]1O
HCID439197

N-Acetyl-Neuraminic Acid

C11H19NO9

结构：CC(=O)OP(=O)([O-])[O-]
HCID4183249

Acetic acid, monoanhydride with phosphoric acid, ion(2-)

C2H3O5P-2

结构：CC(=O)N[C@H]1C([C@H](O)[C@H](O)CO)O[C@](OP(=O)([O-])OC[C@H]2O[C@@H](n3ccc(N)nc3=O)[C@H](O)[C@@H]2O)(C(=O)O)C[C@@H]1O.[Na+]
HCID23679065

未命名化合物

PRODUCTS

生成物

1
结构：CC(=O)N[C@H]1[C@H]([C@H](O)[C@H](O)CO)O[C@](OP(=O)([O-])OC[C@H]2O[C@@H](n3ccc(N)nc3=O)C(O)[C@H]2O)(C(=O)O)C[C@@H]1O.[Na+]
HCID92132133

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

1
结构：Cl.NC(CO)(CO)CO
溶剂HCID93573

Tromethamine hydrochloride

C4H12ClNO3

PROCEDURE

实验过程

A reaction mixture of 20-100 g of 20-80 mM cytidine 5′-monophosphate (CMP, Shanghai QZU Bioscience & Biotechnology), 34.15-136.6 g of 20-80 mM N-acetyl-D-glucosamine (Shanghai Jiubang Chemical), 33.9-101.7 g of 40-120 mM pyruvic acid, 30.9 g of 20 mM MgCl2.H2O (Duksan), 3.72 g of 1 mM CTP (Sigma), 84-525 g of 80-500 mM acetyl phosphate (Sigma) and 7 liters of 50 mM Tris HCl buffer (pH 7.0), maintained at pH 6.5-8.0 using 2M NaOH at 37° C., was mixed with the polymer beads prepared in Example 4, which have each of cytidine monophosphate kinase (CMK), acetate kinase (ACK), NeuAc aldolase (NAN), CMP-NeuAc synthetase (NEU) and GlcNAc-2-epimerase immobilized thereon. Then, the mixture was stirred in a reactor for 5-12 hours.

WORKUP

后处理

  1. temperaturemaintained at pH 6.5-8.0
  2. customat 37° C.
  3. additionwas mixed with the polymer
  4. custombeads prepared in Example 4, which