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A flask equipped with an overhead stirred, thermometer and addition funnel was placed under a nitrogen atmosphere then charged with (E)-hex-2-enoic acid (89.8 g, 787 mmol), EDCI (158.3 g, 826 mmol), HOBt (112.0 g, 826 mmol) and IPAc (890 mL) then cooled to 0±5° C. The addition funnel was charged with NMM (99.1 mL, 1.6 mol) which was then added to the reaction mixture maintaining the temperature at 0±5° C. The mixture was stirred for 30 minutes then cyclopropylamine (60.0 mL, 866 mmol) was added and the reaction allowed to warm to 25±5° C. over 16 hours. The reaction mixture was washed by adding hydrochloric acid (500 mL, 1.0 N) and the mixture stirred vigorously for 30 minutes then allowed to sit for 30 minutes; the layers were separated and the washing procedure repeated. Sodium hydroxide (500 mL, 1.0 N) was added and then the mixture stirred vigorously for 30 minutes then allowed to sit for 30 minutes; the layers were separated and base wash procedure repeated. Water (500 mL) was added and then the mixture stirred vigorously for 30 minutes then allowed to sit for 30 minutes; the layers were separated and the wash procedure repeated. The combined organic phases were concentrated under reduced pressure to ⅓ original volume then IPAc (600 mL) was added; this was repeated two times when a white precipitate formed. The slimy was then concentrated under atmospheric pressure to ⅔ original volume then cooled to 50±5° C. N-heptane (890 mL) was slowly added while the reaction was cooled to −5±5° C. and held at this temperature for 4 hours. The solid was filtered, washed with cold n-heptane (2×250 mL) and dried to provide compound 37 as a fine white solid. 1H NMR (500 MHz, d6-DMSO) 7.89 (s, 1H), 6.58 (dt, J=15.2, 7.0 Hz, 1H), 5.80 (dt, J=15.2, 1.3 Hz, 1H), 2.70-2.65 (m, 1H), 2.12-2.06 (m, 2H), 1.44-1.37 (m, 2H), 0.88 (t, J=7.3 Hz, 3H), 0.64-0.60 (m, 2H), 0.42-0.38 (m, 2H).

1. customA flask equipped with an overhead
2. additionthermometer and addition funnel
3. additionwas then added to the reaction mixture
4. temperaturemaintaining the temperature at 0±5° C
5. stirringThe mixture was stirred for 30 minutes
6. temperatureto warm to 25±5° C. over 16 hours
7. washThe reaction mixture was washed
8. additionby adding hydrochloric acid (500 mL, 1.0 N)
9. stirringthe mixture stirred vigorously for 30 minutes
10. customthe layers were separated
11. additionSodium hydroxide (500 mL, 1.0 N) was added
12. stirringthe mixture stirred vigorously for 30 minutes
13. waitto sit for 30 minutes
14. customthe layers were separated
15. washbase wash procedure
16. additionWater (500 mL) was added
17. stirringthe mixture stirred vigorously for 30 minutes
18. waitto sit for 30 minutes
19. customthe layers were separated
20. concentrationThe combined organic phases were concentrated under reduced pressure
21. additionIPAc (600 mL) was added
22. customformed
23. concentrationThe slimy was then concentrated under atmospheric pressure
24. temperaturethen cooled to 50±5° C
25. additionN-heptane (890 mL) was slowly added while the reaction
26. temperaturewas cooled to −5±5° C.
27. waitheld at this temperature for 4 hours
28. filtrationThe solid was filtered
29. washwashed with cold n-heptane (2×250 mL)
30. customdried