反应详情

50 mg of C-[1-(5-bromo-3-chloro-pyridin-2-yl)-cyclopropyl]-methylamine (step 1 of PREPARATION EXAMPLE 2) was dissolved in 0.9 ml of dichloromethane and 0.085 ml of triethylamine was added at ambient temperature. Then 383 mg of 3-(trifluoromethyl)pyridine-2-carboxylic acid, 54 mg of HOBT hydrate, 76 mg of EDCI.HCl and 37 mg of 3-trifluoromethyl-pyrazine-2-carboxylic acid were added sequentially. The reaction mixture was stirred at ambient temperature for 14 hours and then water was added, the phases were separated and the aqueous phase was extracted with dichloromethane. The organic phases were combined, dried with anhydrous sodium sulphate, filtered and concentrated. Crude material was obtained as a yellow sticky solid, which was purified by flash chromatography on silica gel with cyclohexane/ethyl acetate (1:1) as a solvent. Thus, 76 mg of 3-trifluoromethyl-pyrazine-2-carboxylic acid [1-(5-bromo-3-chloro-pyridin-2-yl)-cyclopropylmethyl]-amide was obtained as a sticky solid. 1H-NMR (CDCl3): 8.80 ppm (s, 1H), 8.75 ppm (s, 1H), 8.65 ppm (s, 1H), 7.95 ppm (s, 1H), 7.70 ppm (m, 1H), 3.72 ppm (d, 2H), 1.18 ppm (m, 4H).

1. customthe phases were separated
2. extractionthe aqueous phase was extracted with dichloromethane
3. dry with materialdried with anhydrous sodium sulphate
4. filtrationfiltered
5. concentrationconcentrated
6. customCrude material was obtained as a yellow sticky solid, which
7. customwas purified by flash chromatography on silica gel with cyclohexane/ethyl acetate (1:1) as a solvent