反应详情

温度

80 °C

A mixture of 4-bromo-2-(2-chloro-6-fluorophenyl)-7-fluoro-2H-pyrazolo[4,3-c]pyridine (172 mg, 0.5 mmol), 4-amino-2,6-dimethylpyrimidine (83 mg, 0.6 mmol), Pd2(dba)3 (11 mg, 0.013 mmol), Xantphos (29 mg, 0.05 mmol) and cesium carbonate (326 mg, 1.0 mmol) in dioxane (5 mL) was de-gassed and purged with nitrogen and the reaction mixture was heated at 80° C. in a sealed vial overnight. The resultant mixture was allowed to cool to room temperature, before being partitioned between ethyl acetate and water. The layers were separated and the organic layer was washed with brine, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by silica gel flash chromatography (80-100% ethyl acetate in cyclohexane). The resultant residue was stirred with HCl (1.25 N in propan-2-ol, 5.0 mL) for 1 hour. The resultant mixture was concentrated under reduced pressure and the solid obtained was triturated with diethyl ether, filtered and dried to afford the title compound as a white solid (153 mg, 70% yield). 1H NMR (400 MHz, DMSO-d6): δ 12.25 (br s, 1H), 9.48 (s, 1H), 8.25 (br s, 1H), 8.15 (d, J=3.6 Hz, 1H), 7.83-7.76 (m, 1H), 7.74-7.66 (m, 2H), 4.70 (s, 2H), 2.67 (s, 3H). LCMS (Method B): RT=2.96 min, m/z: 403 [M+H+].

1. customwas de-gassed
2. custompurged with nitrogen
3. temperatureto cool to room temperature
4. custombefore being partitioned between ethyl acetate and water
5. customThe layers were separated
6. washthe organic layer was washed with brine
7. dry with materialdried over anhydrous sodium sulfate
8. filtrationfiltered
9. concentrationconcentrated under reduced pressure
10. customThe residue was purified by silica gel flash chromatography (80-100% ethyl acetate in cyclohexane)
11. concentrationThe resultant mixture was concentrated under reduced pressure
12. customthe solid obtained
13. customwas triturated with diethyl ether
14. filtrationfiltered
15. customdried