反应详情

温度

4 °C

This phthalimide intermediate (8.0 gram, 0.03 mole) was refluxed in 100 ml. of 6 normal hydrochloric acid overnight. The clear solution was cooled to 4° C., phthalic acid removed by filtration, and the filtrate evaporated to dryness to give a product which, after recrystallization from a mixture of ethanol and ethyl ether, amounted to 4.4 grams of a white solid identified as 4-(3-aminopropyl)-pyrazole dihydrochloride, melting point 189°-190° C. This pyrazole compound could be coupled to the activated dextran by reaction of the terminal amino group, but the resulting affinity resin, in which the coupling arm had a length of only 6 Angstrom units, had poor binding capacity for the binary complex of alcohol dehydrogenase and NAD.

1. customphthalic acid removed by filtration
2. customthe filtrate evaporated to dryness
3. customto give a product which
4. customafter recrystallization
5. additionfrom a mixture of ethanol and ethyl ether
6. customThis pyrazole compound could be coupled to the activated dextran by reaction of the terminal amino group