反应详情

To a solution of (S,Z)-2-(((1-(benzhydryloxy)-3-(4-(N-(1-(tert-butoxycarbonyl)piperidin-4-yl)carbamimidoyl)phenoxy)-1-oxopropan-2-yl)oxy)imino)-2-(2-((tert-butoxycarbonyl)amino)thiazol-4-yl)acetic acid (Prepared according to WO2013110643, 72 mg, 0.085 mmol), (3S,4R)-4-((1H-1,2,4-triazol-1-yl)methyl)-3-aminoazetidin-2-one (15.7 mg, 0.094 mmol) and HATU (42.2 mg, 0.111 mmol) in DMF (854 μL) was added DIPEA (44.8 μL, 0.256 mmol). After stirring at for 3 h it was diluted with EtOAc, washed with water, NaHCO3 (aq satd), brine, dried over Na2SO4 and concentrated in vacuo, affording the title compound (82 mg, 87%) as an olive film. LCMS: Rt=0.97 min, m/z=992.5 (M+1) Method 2m_acidic.

1. washwashed with water, NaHCO3 (aq satd), brine
2. dry with materialdried over Na2SO4
3. concentrationconcentrated in vacuo