Bromoacetic Acid → HCID 23568317, compound

HRID 77274Notes

Reaction equation

HRID 77274 reaction equation
CN([CH:4]=[O:5])C.[Br:6][CH2:7][C:8]([OH:10])=O.C(=O)([O-])[O-:12].C1N=C(N)C2N=CN([C@@H]3O[C@H](COP([O:34][P:35]([O:38][CH2:39][C@H:40]4[O:44][C@@H:43](N5C=C(C(N)=O)CC=C5)[C@H:42]([OH:54])[C@@H:41]4[OH:55])([OH:37])=[O:36])(O)=O)[C@@H](O)[C@H]3O)C=2N=1>C(=O)(O)O.P(O)(O)(O)=O>[P:35]([O:38][CH2:39][C@@H:40]([OH:44])[C@@H:41]([OH:55])[C@H:42]([OH:54])[C@@H:43]([OH:12])[C:4]([C:8](=[O:10])[CH2:7][Br:6])=[O:5])([OH:34])([OH:37])=[O:36]

Participants

6

Reactant 4

HCID 6227Bromoacetic Acid3×HCID 6228DimethylformamideHCID 19660Carbonate IonHCID 439153Nadh

Product 1

HCID 23568317HCID 23568317, compound

Reagents, catalysts & solvents 1

HCID 23221470Phosphate carbonateSolvent

Procedure

100 μL DMF was added to bromoacetic acid NHS (Sigma 3.06 mg, 12.97 μM) and stirred. A 2.0 mL (10 mg/mL) G6PDH solution was prepared in 0.025 M phosphate carbonate buffer, pH 7.2 and adjusted to pH 8.5 with 0.4 M carbonate buffer. 45 mg disodium G6P and 90 mg NADH, was dissolved in the G6PDH solution. Bromoacetic acid NHS was added to G6PDH solution at 5 μL increments. Enzyme activity was measured on the HITACHI 917 analyzer after each addition. Bromoacetic acid NHS was added until approximately 63.0% enzyme deactivation was obtained. G6PDH conjugation solution was dialyzed with 3×4 liter portions of 0.01 M phosphate, pH 7.2.

Workup

  1. additionafter each addition
  2. customwas obtained
  3. customG6PDH conjugation solution was dialyzed with 3×4 liter portions of 0.01 M phosphate, pH 7.2

Sources & evidence

ORD record
ord-0274af0b70ec488fbec84225ba54e218
Source dataset
uspto-grants-2014_09
Patent number
US08841136B2

Reaction notes

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