L-Phenylalanine → 2-Propenoic acid, 3-phenyl-

HRID 847133Notes

Reaction equation

HRID 847133 reaction equation
C(O)C(N)(CO)CO.Cl.N[C@H:11]([C:19]([OH:21])=[O:20])[CH2:12][C:13]1[CH:18]=[CH:17][CH:16]=[CH:15][CH:14]=1>>[C:19]([OH:21])(=[O:20])[CH:11]=[CH:12][C:13]1[CH:14]=[CH:15][CH:16]=[CH:17][CH:18]=1

Participants

3

Reactant 2

HCID 6140L-PhenylalanineHCID 93573Tromethamine hydrochloride

Product 1

HCID 87842-Propenoic acid, 3-phenyl-

Procedure

First, a sample of a cell extract was diluted with a 25 mM Tris-HCl buffer solution (pH 8.8) to give a cell concentration of about 1% by wet weight and a 1.0 ml portion thereof was added to 4.0 ml of a 31.25 mM Tris-HCl buffer solution (pH 8.8) supplemented with 31.25 mM L-phenylalanine. The resultant solution was allowed to react at 30° C. for 20 minutes and then the reaction was terminated by addition of 1 ml of 1N-HCl. The amount of cinnamic acid produced in the reaction mixture was determined by liquid chromatography under the conditions as hereinafter described to estimate the PAL activity.

Workup

  1. extractionFirst, a sample of a cell extract
  2. additionwas diluted with a 25 mM Tris-HCl buffer solution (pH 8.8)
  3. customto give a cell concentration of about 1% by wet weight
  4. customto react at 30° C. for 20 minutes
  5. customthe reaction was terminated by addition of 1 ml of 1N-HCl

Sources & evidence

ORD record
ord-9b0312ef271d4474b2122b3d24d90b45
Source dataset
uspto-grants-1992_08
Patent number
US05139935

Reaction notes

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