反应详情

HOBt (81 mg, 0.6 mmole) and EDC (105 mg, 0.55 mmole) are added to 1-carboxy-5-piperdinylglutaramide (IX, 100 mg, 0.5 mmole) in DMF (2 ml). The acid is activated 60 minutes then treated with 1-9H-fluoren-9-ylmethyl (2R,3S)-3-amino-4-(3,5-difluorophenyl)-2-hydroxybutyl(3-iodobenzyl)carbamate hydrochloride (XXXV, PREPARATION 14, 300 mg, 0.43 mmole) and NMM (0.19 ml, 1.72 mmole). The reaction is stirred 3 hrs then concentrated under reduced pressure. The residue is partitioned between ethyl acetate and saturated aqueous bicarbonate. The organic phases are washed with aqueous potassium bisulfate, saline, dried over sodium sulfate and finally concentrated under reduced pressure to give crude product. Purification via flash chromatography with ethyl acetate/hexane (50/50) then methanol/ethyl acetate (10/90) gives the title compound, M+H=838 g/m.

1. concentrationthen concentrated under reduced pressure
2. customThe residue is partitioned between ethyl acetate and saturated aqueous bicarbonate
3. washThe organic phases are washed with aqueous potassium bisulfate, saline
4. dry with materialdried over sodium sulfate
5. concentrationfinally concentrated under reduced pressure
6. customto give crude product
7. customPurification via flash chromatography with ethyl acetate/hexane (50/50)