Potassium Carbonate → 2-(4-(((Benzyloxy)carbonyl)amino)-2-oxopyrimidin-1(2H)-yl)acetic acid

Temperature

0 °C

In a three-necked round bottom flask equipped with mechanical stirring and nitrogen inlet was placed methyl bromacetate (7.82 mL, 82.6 mmol) and a suspension of N4 -benzyloxycarbonyl-cytosine (9, 21 g, 82.6 mmol) and potassium carbonate (11.4 g, 82.6 mmol) in dry DMF (900 mL). The mixture was stirred vigorously overnight, filtered, and evaporated to dryness, in vacuo. Water (300 mL) and 4N hydrochloric acid (10 mL) were added, the mixture was stirred for 15 minutes at 0° C., filtered, and washed with water (2×75 mL). The isolated precipitate was treated with water (120 mL), 2N sodium hydroxide (60 mL), stirred for 30 minutes, filtered, cooled to 0° C., and 4N hydrochloric acid (35 mL) was added. The title compound was isolated by filtration, washed thoroughly with water, recrystallized from methanol (1000 mL) and washed thoroughly with ether. This afforded 7.70 g (31%) of pure title compound. The mother liquor from recrystallization was reduced to a volume of 200 mL and cooled to 0° C. This afforded an additional 2.30 g of a material that was pure by tlc but had a reddish color. M.p. 266°-274° C. Anal. for C14H13N3O5. Found(calc.); C: 55.41(55.45); H: 4.23(4.32); N: 14.04(13.86). 1H-NMR (90 MHz; DMSO-d6): 8.02 ppm (d,J=7.32 Hz, 1H, H-6); 7.39 (s, 5H, Ph); 7.01 (d, J=7.32 Hz, 1H, H-5); 5.19 (s, 2H, PhCH2 --); 4.52 (s, 2H).

1. customIn a three-necked round bottom flask equipped
2. customnitrogen inlet was placed
3. stirringThe mixture was stirred vigorously overnight
4. filtrationfiltered
5. customevaporated to dryness, in vacuo
6. additionWater (300 mL) and 4N hydrochloric acid (10 mL) were added
7. stirringthe mixture was stirred for 15 minutes at 0° C.
8. filtrationfiltered
9. washwashed with water (2×75 mL)
10. additionThe isolated precipitate was treated with water (120 mL), 2N sodium hydroxide (60 mL)
11. stirringstirred for 30 minutes
12. filtrationfiltered
13. addition4N hydrochloric acid (35 mL) was added

ORD record

ord-0925e3e6b21c4c29bda59ab05aef949d

Source dataset

uspto-grants-1998_02

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05714331