Sucrose → 2-(N-Morpholino)ethanesulfonic acid hydrate

HRID 909988Notes

Reaction equation

HRID 909988 reaction equation
C(O)[C:2](N)(CO)[CH2:3][OH:4].[Cl-].[Cl-].[Ca+2].C[C@@H]([C@@H]1[C@@]2(C)[C@@H](O)C[C@@H]3[C@@]4(C)CC[C@@H](O)C[C@H]4C[C@@H](O)[C@H]3[C@@H]2CC1)C[CH2:15][C:16]([NH:18][CH2:19][CH2:20][S:21]([O-:24])(=[O:23])=[O:22])=[O:17].[Na+].C(O)[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O>O>[CH2:2]1[N:18]([CH2:19][CH2:20][S:21]([OH:24])(=[O:22])=[O:23])[CH2:16][CH2:15][O:4][CH2:3]1.[OH2:17]

Participants

6

Reactant 4

HCID 5988SucroseHCID 6503Tris(hydroxymethyl)aminomethaneHCID 5284359Calcium Chloride2×HCID 23666345Sodium Taurocholate

Product 1

HCID 162184172-(N-Morpholino)ethanesulfonic acid hydrate

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Prelysis solution: The solution is prepared according to Graves, L, et al. (1993). Universal Bacterial DNA isolation procedure. In Diagnostic molecular microbiology: principles and applications, D. Persing, T. Smith, F. Tenover and T. White, eds. (Washington, D.C.: American Society for Microbiology). The solution, which is freshly prepared and maintained on ice, contains: 0.25 ml of 2M Tris (pH 7.0), 3.1 ml of pancreatic lipase (prepared by dissolving 6.1 mg of pancreatic lipase (Sigma) in 59.8 ml of water and adding 1.2 ml of 0.1M CaCl2; stored at −20° C. in 3.1 ml aliquots), 0.3 ml of 1% sodium taurocholate (Difco), 0.5 ml of 0.1M CaCl2, 5.25 ml of sucrose, and 0.05 g of lysozyme.

Workup

  1. customPrelysis solution: The solution is prepared
  2. customThe solution, which is freshly prepared
  3. temperaturemaintained on ice

Sources & evidence

ORD record
ord-f7f90e3043af4b2fb80023256c47ad4f
Source dataset
uspto-grants-2011_09
Patent number
US08021848B2

Reaction notes

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