Sodium Chloride → L-Ascorbic Acid

HRID 923824Notes

Reaction equation

[OH:1][CH2:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].OCC([C@H]([C@@H]([C@H](CO)O)O)O)=O.[Na+].[Cl-].C([O-])([O-])=O.[Ca+2]>>[OH:8][C:7]1[C@@H:5]([C@@H:3]([OH:4])[CH2:2][OH:1])[O:6][C:11](=[O:12])[C:9]=1[OH:10]

Participants

Reactant 4

HCID 5234Sodium Chloride

HCID 5780Sorbitol

HCID 6904L-(-)-Sorbose

HCID 10112Calcium Carbonate

Product 1

HCID 54670067L-Ascorbic Acid

Procedure

A series of resting cell reactions (0.5 ml reaction mixture in 5 ml reaction tube) was carried out with 2% D-sorbitol or with 2% L-sorbose, and all reaction mixtures further contained 0.3% NaCl, 1% CaCO3 and cells at a final concentration of 5 absorbance units at 600 nanometers (OD600). After 20 h incubation time, G. oxydans DSM 17078 produced 270 mg/l or 670 mg/l of Vitamin C, respectively from 2% D-sorbitol or 2% L-sorbose, respectively. In comparison, strain G. oxydans DSM 17078-SMS 05::Km produced 1540 mg/l or 1990 g/l of Vitamin C, respectively.

Workup

customcell reactions (0.5 ml reaction mixture in 5 ml reaction tube)
customall reaction mixtures

Sources & evidence

ORD record: ord-0426bfd8bcbe40eda3dc68eddd4b04ff
Source dataset: uspto-grants-2016_03
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US09279138B2

Reaction notes

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