5'-Atp → Adenosine Monophosphate

HRID 965471Notes

Reaction equation

HRID 965471 reaction equation
[P:1]([O:9][CH2:10][C@H:11]1[O:15][C@@H:14]([N:16]2[C:25]3[N:24]=[CH:23][N:22]=[C:20]([NH2:21])[C:19]=3[N:18]=[CH:17]2)[C@H:13]([OH:26])[C@@H:12]1[OH:27])([O:4]P(O)(O)=O)(=[O:3])[OH:2].P(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O.OP([O-])(O)=O.OP([O-])([O-])=O.[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+].C(O)C(N)(CO)CO.Cl.[Mg]>>[CH:23]1[N:22]=[C:20]([NH2:21])[C:19]2[N:18]=[CH:17][N:16]([C@@H:14]3[O:15][C@H:11]([CH2:10][O:9][P:1]([OH:4])([OH:3])=[O:2])[C@@H:12]([OH:27])[C@H:13]3[OH:26])[C:25]=2[N:24]=1

Participants

6

Reactant 5

HCID 59575'-AtpHCID 60225'-Adp2×HCID 93573Tromethamine hydrochlorideHCID 5462224MagnesiumHCID 24978514Phosphate Buffered Saline

Product 1

HCID 6083Adenosine Monophosphate

Procedure

A second embodiment of the luminometer of the present invention is provided wherein an additional reagent flow is included for feeding very pure adenosine diphosphate (ADP) reagent, with respect to ATP, into the processing fluid downstream of the junction (5) where detergent is added, but upstream of the luminometer. The ADP reagent (99.95% pure with respect to ATP) is added at a flow and concentration such as to produce a concentration of 0.5 mM in the final flow. The processing fluid is pH 7.4 phosphate buffered saline or TrisHCl pH 7.8 including 0.2 mM magnesium ions.

Workup

  1. customA second embodiment of the luminometer of the present invention is provided wherein an additional reagent flow
  2. customto produce a concentration of 0.5 mM in the final flow

Sources & evidence

ORD record
ord-f46e2aa47c954c5db35f79c7548b9286
Source dataset
uspto-grants-1998_06
Patent number
US05773710

Reaction notes

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