HRID965471

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O

HCID5957

Adenosine triphosphate

C10H16N5O13P3

结构：Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O

HCID6022

Adenosine diphosphate

C10H15N5O10P2

2 次

HCID93573

Tromethamine hydrochloride

C4H12ClNO3

HCID5462224

Magnesium

结构：O=P([O-])(O)O.O=P([O-])([O-])O.[Cl-].[Cl-].[K+].[K+].[Na+].[Na+].[Na+]

HCID24978514

未命名化合物

PRODUCTS

生成物

结构：Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)O)[C@@H](O)[C@H]1O

HCID6083

Adenosine 5'-phosphate

C10H14N5O7P

PROCEDURE

实验过程

A second embodiment of the luminometer of the present invention is provided wherein an additional reagent flow is included for feeding very pure adenosine diphosphate (ADP) reagent, with respect to ATP, into the processing fluid downstream of the junction (5) where detergent is added, but upstream of the luminometer. The ADP reagent (99.95% pure with respect to ATP) is added at a flow and concentration such as to produce a concentration of 0.5 mM in the final flow. The processing fluid is pH 7.4 phosphate buffered saline or TrisHCl pH 7.8 including 0.2 mM magnesium ions.

WORKUP

后处理

customA second embodiment of the luminometer of the present invention is provided wherein an additional reagent flow
customto produce a concentration of 0.5 mM in the final flow