反应详情

The inclusion complex (100 g) of clavulanic acid with heptakis-(2,3,6-tri-O-acetyl)-β-CD, which was prepared in the same way as in Example 4, was suspended in a 3% aqueous potassium carbonate solution (200 ml), in which methyl-β-CD (53.6 g, 40.9 mmol) was dissolved. The suspension was stirred for 5 min at ambient temperature, the solid particles were filtered off and the filtrate was frozen in liquid nitrogen and then freeze-dried. There was obtained a yellowish microcrystalline inclusion complex (56.7 g, 91.9%) of potassium clavulanate with methyl-β-CD. The complex contained 14.9% of potassium clavulanate as it was determined by the HPLC method.

1. dissolutionwas dissolved
2. filtrationthe solid particles were filtered off
3. temperaturethe filtrate was frozen in liquid nitrogen
4. customfreeze-dried