反应详情

Portions of 3-bromo-2-(3-fluoro-phenyl)-6-methoxy-pyrazolo[1,5-b]pyridazine (400 mg, 2.1 g total) were placed in individual Reactivials equipped with a magnetic stirrer bar. Pyridine hydrochloride (10 eq) was added to each vial, the vials sealed, and heated to 200° for 3 hours. The vials were allowed to cool to ˜140° before opening and the contents poured into ice/water. The resulting mixture was extracted into ethyl acetate (3×100 ml) and the combined organic extracts washed with water (7×75 ml), dried (Na2SO4) and evaporated to give the des-bromo phenol as a brown solid (1.0 g, MH+ 230). This solid was dissolved in anhydrous DMF (10 ml) and sodium hydride (60% dispersion in mineral oil, 200 mg) added portionwise. After stirring for 20 minutes at ambient temperature the solution was transferred to a small cooled autoclave and bromodifluoromethane (5 ml, xs, condensed at −30°) added. The autoclave was then sealed, allowed to warm to ambient temperature and stirred for 36 hours. The resulting solution was diluted with ethyl acetate (200 ml), washed with water (10×20 ml), dried (Na2SO4), concentrated and the residual gum purified by flash column chromatography with cyclohexane:ethyl acetate (4:1) as eluant. This gave the title compound as a solid (652 mg, 60%).

1. customequipped with a magnetic stirrer bar
2. customsealed
3. temperatureheated to 200° for 3 hours
4. temperatureto cool to ˜140° before opening
5. extractionThe resulting mixture was extracted into ethyl acetate (3×100 ml)
6. washthe combined organic extracts washed with water (7×75 ml)
7. dry with materialdried (Na2SO4)
8. customevaporated
9. customto give the des-bromo phenol as a brown solid (1.0 g, MH+ 230)
10. additionadded
11. customThe autoclave was then sealed
12. temperatureto warm to ambient temperature
13. stirringstirred for 36 hours
14. washwashed with water (10×20 ml)
15. dry with materialdried (Na2SO4)
16. concentrationconcentrated
17. customthe residual gum purified by flash column chromatography with cyclohexane:ethyl acetate (4:1) as eluant